Click on the image to the left to view a virtual slide comparison of an adult wild-type (Connor) and an adult golden (gol) mutant.

Mutant image (bottom) is of a 7dpf huli hutu (hht), ENU-generated mutation currently being fine mapped (in-house). Normal image (top) is of a comparable 7dpf wild-type.

• hht causes striking architectural and cytologic changes in several organs
• The eyes of a variable fraction of mutants are reduced in size
• Cell types of different retinal layers are mixed together to varying degrees
• The eye phenotype is detectable at 2dpf and can be mild to severe at 7dpf
• Severe cytologic atypia is seen in the intestine, pancreas, liver, swim bladder, and pneumatic duct
• This atypia includes a great variability in nuclear size and shape, frequent multinucleation, and architectural disorganization

  from Mohideen, et al "Histology-based screen for zebrafish mutants with abnormal cell differentiation" in Dev Dyn 228:414-423 2003.

Micro CT

Top: A screen-capture of part of a 3D model generated from these 1500 2D slices in a Volume Graphics software package known as VGStudio Max.

Middle: One of 1500 digital, coronal 2D slices generated by applying the Feldkamp cone-beam reconstruction algorithm to these 600 x-rays.

Bottom: One of 600 x-rays taken through 180° of the head of an unstained, 60 dpf juvenile zebrafish (one x-ray every 0.3°).

Top: a specimen bearing a tumor on the right side of the section. Age: unknown, but older than the bottom fish.

Bottom: a healthy specimen